Population of Shicaridae invading Bangkok, Thailand

Mediterranean recluse spider, Loxoceres rufescensIt was first discovered living in a human habitation in Bangkok, Thailand. The survey across 39 regions revealed five facilities: L. rufescens population. The highest density was recorded at a warehouse on Yaowarat Road in the heart of Bangkok’s Chinatown, where 315 spiders, including adults, juveniles and baby spiders, were found. The presence of medically important spiders in such densely populated urban areas raises concerns about potential poisoning risks. 13 specimens L. rufescens DNA was extracted and sequenced for molecular phylogenetic analysis. COI and ITS2 markers were used to investigate internal relationships. L. rufescens and in the available area Loxoceles Seed array. The results show that COI is better at resolving species-level genetic clusters compared to ITS2. Surprisingly, L. rufescens Individuals from the same family have been found in COI lineages that are quite distant, suggesting that mtDNA may not be suitable for studying intraspecific phylogeography in this case. The species boundary methods ABGD and ASAP showed promising results for both COI and ITS2, whereas bPTP and GMYC tended to overestimate the number of species. ITS2 showed high sequence similarity. L. rufescenssuggesting its potential utility as a barcode marker for identifying this globally distributed species. Genetic distance analysis revealed a potential barcoding gap (K2P) of 8–9% for COI and less than 2% for ITS2. Loxoceles. This study contributes valuable sequence data for a medically important genus. Loxoceles and emphasizes the need for an integrated approach in understanding its evolution and spread. This finding has important implications for pest management strategies and public health in urban environments.