Mass spectrometry revealed biological macromolecular components of medicinal scorpion (Buthus martensii Karsch) and proteomic characteristics for various applications.

Abstract

A total of 17605 gene sequences were obtained from transcriptome sequencing, and 12,368 predictable proteins, 278 transcription factors, and 51 transcription factor family information were obtained from database comparison. In the process of proteome identification, 10072 peptides were found, and 1917 protein components were deduced from peptides. In the process of peptide identification, 6758 peptides were matched with 33834 peptides in the transcriptome database. In addition, protein de novo sequencing was used to detect protein changes of whole scorpion tail and whole scorpion tail removal in bulk and tonic. In peptidome identification, 6758 peptides were found in the transcriptome database, and 33834 peptides were identified by protein de novo sequencing. In addition, the changes in the proteins of scorpion tail and scorpion tail in powder and decoction were investigated, and it was found that different sites of drugs and drug administration methods may bring about changes in the function of scorpion tail. For example, when scorpion is powdered and made into a drug in bulk, the protein components are more completely preserved, and its efficacy is closely related to inflammatory responses and signal transduction. In decoction, the protein components are broken down and hydrolyzed into amino acids and peptide segments, and its efficacy is closely related to the regulation of amino acids and the maintenance of functions such as energy.