Rosa Pineda -Own Work, CC BY -SA 3.0, https://commons.wikimedia.org/w/index.php?curid=27718135
Inhibitory ability of three scorpion venom peptides against multidrug-resistant Klebsiella pnemoniae.
background:
Increased bacterial infections caused by drug-resistant bacterial infections, especially those caused by Klebsiella pneumoniae It highlights the urgent need for new treatment candidates. From infections at hospitals K. Pneumonia Carbapenemase (KPC) – Producing bacteria pose serious health threats, particularly affecting the respiratory and urinary tract. This study investigates the antibacterial effects of three antibacterial peptides called pantinin, derived from. Pandinas Impeller Against scorpion poison K. Pneumonia Various KPC-producing clinical isolates.
method:
Non-toxic concentrations were established for each peptide using the MTT method. Antibacterial activity was performed via liquid microdilution assays to assess minimum inhibitory concentration (MIC) and minimal sterilization concentration (MBC). A time kill test was performed several times by recording colony-forming units (CFUs) and comparing them with those of untreated bacteria. Finally, the effect of peptides on gene expression of pathogenic factors of K. Pneumonia It was assessed via QPCR.
result:
The antibacterial assay showed the bactericidal effects of pantinin-1 and pantinin-2 within 1 hour, and pantinin-3 exhibited bacterial properties. Notable, microphone values ​​ranged from 6-25μm. K. Pneumonia For KPC strains, it is 25-50 μm. Pantinin exerts antibacterial effects through membrane degradation mechanisms and interacts directly with outer membrane lipopolysaccharide (LPS), as evidenced by cyclic dichroism (CD) spectra and scanning electron microscopy (SEM). Furthermore, QPCR shows a decrease in pathogenic gene expression, suggesting the antibacterial effect of the peptide at transcriptional levels.
Conclusion:
These findings support Pantinin as a promising candidate for treating multidrug resistance K. Pneumonia infection.
Giugliano, R., Della Marca, R., Chianese, A., Monti, A., Donadio, F., Esposito, E., Doti, N., Zannella, C., Galdiero, M. , and De Filippis, A. (2025). Inhibitory ability of three scorpion venom peptides against multidrug-resistant Klebsiella pnemoniae. Frontiers of Microbiology, 161569719. https://doi.org/10.3389/fmicb.2025.1569719
