Here we report the innovative application of whole-cell patch clamp electrophysiology to assess broad-spectrum neutralization of medically important Scorpion venoms by three antivenoms. Centroides. Damage caused by 21 different types of poisons has been reported. Centruroides As many as 300,000 envenoming cases occur annually in Mexico, representing a severe and potentially life-threatening pathophysiology. In vitro Expression of envenomation against two human voltage-dependent sodium (hNa)Five) Channel subtype: hNaFive1.4 and hNaFive1.5 is expressed primarily in skeletal and cardiac muscle cells. We next characterized the neutralization activity of three different antivenoms using a direct competition model against the more potent target, hNa.Five1.4. Widespread neutralization was observed, but neutralization was variable. Centroroides elegans, C. limpidus, C. Noxius and C. safsus Despite the presence of large numbers of these toxins in the immune mixture, the antitoxin is not neutralized, raising questions about the truly “broad” neutralizing ability of the antitoxin. This study not only extends previous testing of the antitoxin, In vitro This study is not only an investigation of antivenom efficacy using whole-cell patch clamp techniques, but also highlights the potential of this animal-free model in terms of cross-reactivity, experimental scalability, and most importantly, informing clinical management practice regarding antivenom administration in Mexico.
Campbell, SI, Chow, CY, Neri-Castro, E., Alagón, A., Gómez, A., Soria, R., King, GF, & Fry, BG (2024). Removing scorpion stings: an electrophysiological investigation of the relative efficacy of three antivenoms against the medically important genus Centruroides. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 283109977. https://doi.org/10.1016/j.cbpc.2024.109977
