Nosocomial infections due to antibiotic-resistant bacteria cause significant economic losses and human health problems, including acute lung injury. The aim of this study was to investigate the role of scorpion venom in combating lung injury caused by bacterial sepsis. Androctonus crassicauda (Acra) scorpion venom was fractionated by HPLC and tested against bacteria. The antibacterial fraction (AcraX) obtained at 36 min was used to produce chitosan-coated particles. Rats were administered venom at 6 h after induction of sepsis and sacrificed 24 h later. Lung tissue was used for histopathological analysis and, after homogenization, for cytokine (TNF-α, IL-1β, IL-6, IL-10) measurements by ELISA. Immunohistochemistry was performed for extracellular matrix and fibrosis using anti-MMP-2 and MMP-9 antibodies. AcraX (Staphylococcus aureus, Bacillus pneumoniae, A. Baumani, and P. aeruginosa), a positive result is Klebsiella pneumoniaeThe regenerative effect of the venom on the lesions induced in tissues and HeLa cell line was also observed. HeLa cell line exposed to the venom achieved 100% viability. This was also observed histologically through changes in the alveolar septa in lung tissue sections, reduction in the inflamed areas and hyaline membrane structures in the septic group. In conclusion, Akula venom acts as an effective anti-inflammatory agent against inflammation. Klebsiella pneumoniae It caused pneumonia.
Oskay, B., Sahin, K., Caliscan, F. etc. Chitosan-coated scorpion venom Klebsiella pneumoniae. International Journal of Peptide Research 3054 (2024). https://doi.org/10.1007/s10989-024-10635-5
