Genetic barcoding for species identification and phylogenetic estimation of ghost spiders (Arachnidae: Arachnidae: Amaurobiodinae)
abstract
Identification of spider species poses many challenges, as in most cases the traits used are derived from reproductive structures that are fully developed only in the adult stage, and identification of immature spiders is almost always impossible. . Furthermore, these structures usually also exhibit some degree of intraspecific variation, in some cases making identification of closely related species difficult. Genetic barcoding technology (DNA barcoding) based on sequencing of mitochondrial markers, cytochromes. c Oxidase subunit I (COI) has proven to be a useful complementary tool to overcome these limitations. In this study, we investigate the contribution of DNA barcoding to the taxonomy of the subfamily Amaurobiodinae using a sophisticated single linkage analysis (RESL) algorithm for operational taxonomic unit (OTU) delimitation. , compared with the assembled species by automatic segmentation (ASAP). ) algorithm and the other three markers (28S rRNA, 16S rRNA, histone H3), in addition to COI. Of the total 97 included species identified by morphology, 82 matched the operational taxonomic units obtained from RESL, indicating 85% concordance between the two methods. Similar results were obtained using the ASAP algorithm. Confirming previous observations of morphological variation within the same species, this technique provides new information about the genetic structure and potentially enigmatic species. Most of the discrepancies between DNA barcoding and morphological identification are explained by low geographic sampling or lineage divergence or geographically structured lineages. After adding many specimens using only COI In our data, multimarker phylogenetic analysis is consistent with previous results and provides improved support. marker COIimmediately followed 28Sthe most phylogenetically informative. We believe that barcode DNA technology, together with morphological and geographic data, is a valuable data source for identifying Amaurobiodinae species, and that there are cases that require more detailed and in-depth study. We conclude that it is also useful for detection.
Barone Mariana L., Wilson Jeremy D., Zapata Lorena, Soto Eduardo M., Haddad Charles R., Grismad Cristian, Izquierdo Matias, Arias Elizabeth, Pizarro-Araya Jaime, Briones Raúl, Barriga Juan Enrique, Peralta Luciano, RamÃrez MartÃn J. (2024) Genetic barcoding for species identification and phylogenetic inference of ghost spiders (Arachnidae: Amaurobiodinidae). Invertebrate systematics 38, IS24053.
